黄芪对高脂饲养大鼠脂肪组织分布的影响

Effect of radix astragali (RA) on the fat distribution of rat with high-fat-diet

目的 :探讨黄芪改善高脂饮食大鼠胰岛素敏感性的机制。方法 :高脂饲养大鼠 ,治疗组同时灌服黄芪 ,对照组不灌服 ,另设正常饮食之正常对照组。采用葡萄糖 -胰岛素耐量实验测量大鼠胰岛素敏感性 (K值 ) ,并观察不同部位脂肪组织重量的变化及其与胰岛素敏感性 (K值 )的关系。结果 :7周后 ,对照组大鼠K值减小 ,腹腔脂肪组织重量增加 ;治疗组大鼠经黄芪治疗后K值较正常组有所减小 ,但与对照组相比明显增大 ;治疗组大鼠腹腔脂肪组织重量未见增加 ,与对照组相比有明显差异 (P <0 .0 1) ,与正常对照组相比未见明显差异 (P >0 .0 5 )。结论 :黄芪具有改善胰岛素抵抗的作用 ;黄芪改善高脂饮食大鼠胰岛素敏感性的机制可能与调控脂肪细胞分布有关。

Objective: To investigate the mechanism of radix astragali (RA), by which insulin resistance (IR) in adipose tissue of the rat with high-fat-diet (HF) is enhanced. Methods: The SD rats were randomly divided into 3 groups: (1) the HF group, i.e. rats with high-fat diet (fat 61% of total kcal); (2) the HF + RA group, supplemented with high-fat-diet plus radix astragali; (3) the control group, i.e. the normal diet group. Each group was fed equicalorically for 4 weeks, then insulin sensitivity was measured with glucose-insulin tolerance test. The adipose tissue in subcutanea and retroperitonea was weighed. Results: (1) K values obtained from glucose-insulin tolerance test were significantly increased in the HF + RA group compared with that of the HF group. (2) Fat weight in the same position in retroperitonea was significantly increased in the HF group compared with that of HF + RA group. Conclusion: Treatment with RA can relieve insulin resistance of the rat with high-fat-diet. The mechanism of radix astragali, which relieve insulin resistance in adipose tissue of the rat with high-fat-diet might be correlated with body fat distribution, though it is still unknown why RA treatment can decrease adipose tissue in retroperitonea.