乙酰肝素酶基因在子宫内膜异位症患者在位与异位子宫内膜组织中的表达

Expression of heparanase gene in eutopic and ectopic endometrium of women with endometriosis

目的 研究乙酰肝素酶基因在子宫内膜异位症发病中的作用。方法 应用原位杂交技术 ,检测腹腔镜手术中证实为子宫内膜异位症患者 (EM组 ,2 3例 )的在位和异位子宫内膜组织乙酰肝素酶mRNA的表达 ,并与正常妇女 (对照组 ,2 5例 )子宫内膜组织比较。结果 ( 1)EM组在位与异位子宫内膜乙酰肝素酶mRNA阳性表达率一致 ,但异位子宫内膜组织中 ,阳性细胞着色较深。在位子宫内膜腺上皮细胞较间质细胞胞浆染色深 ,异位子宫内膜腺上皮细胞与间质细胞胞浆着色基本一致 ;EM组增生期与分泌期子宫内膜均有乙酰肝素酶mRNA的表达 ,增生期阳性表达率为 83 3%( 10 / 12 ) ;分泌期为 72 7% ( 8/ 11) ,两者比较 ,差异也无显著性 (P >0 0 5 ) ;( 2 )对照组增生期子宫内膜乙酰肝素酶mRNA的阳性表达率为 4 1 7% ( 5 / 12 ) ,分泌期子宫内膜阳性表达率为 7 7% ( 1/ 13) ,两者比较 ,差异有显著性 (P <0 0 5 )。 ( 3)EM组子宫内膜乙酰肝素酶mRNA阳性表达率为 78 3% ( 18/2 3) ;对照组为 2 4 0 % ( 6 / 2 5 ) ,两者比较 ,差异也有显著性 (P <0 0 5 )。结论 乙酰肝素酶基因的表达与子宫内膜异位症的发病相关 ,其可能成为子宫内膜异位症治疗的一个有价值的靶点。

Objective To study the function of heparanase gene in the pathogenesis of endometriosis Methods In situ hybridization method was used to examine the heparanase mRNA expression in the eutopic and ectopic endometrium of patients with endometriosis(EM group, 23) and the eutopic endometrium of women without endometriosis(control group,25) Results (1) In EM group: The frequency of heparanase gene expression in eutopic was similar to that in ectopic endometrium However the number of positive cells and the intensity of heparanase gene expression in ectopic endometrium was more than that in eutopic endometrium The frequency of heparanase gene expression in proliferative phase was 83 3%(10/12), while in secretory phase it was 72 7%(8/11), the difference is not significant; (2) In control group: The frequency of heparanase gene expression in proliferative phase was 41 7%(5/12), while in secretory phase it was 7 7%(1/13), the difference is significant; (3) The frequency of heparanase gene expression in endometrium of patients with endometriosis and women without endometriosis were 78 3%(18/23) and 24.0%(6/25) respectively, the difference is significant Conclusions Heparanase gene may play some roles in the pathogenesis of endometriosis, and it may participate the regulation of menstrual cycle, and that it may participate the regulation of menstrual cycle It may be an important target of the trentment for endometriosis