铝-铬天青S-Triton X-100光度法测定蛋白质(英文)

Spectrophotometric Method for the Determination of Proteins with Aluminum-Chrome Azurol S-Triton X-100 Complex

在pH 3 0～ 4 3的酸性介质中 ,铝 铬天青S TritonX 10 0配合物与蛋白质迅速反应生成多元配合物 ,从而引起吸收光谱的改变 ,在 2 2 0nm和 6 36nm附近吸光度增大 ,且吸光度差ΔA(=A0 -A)值与蛋白质的浓度成正比 .不同蛋白质在 0～ 5 0mg/L和 10～ 80mg/L范围内遵循比尔定律 ,各反应的摩尔吸光系数分别在 4 2 3× 10 5～ 2 0 1× 10 6(2 2 0nm附近 )和 2 6 4× 10 5～ 1 6 4× 10 6(6 36nm附近 )之间 .基于此 ,建立了一种测定蛋白质的新光度法 .该法简便、快速、选择性好 ,用于人血清和尿液样品中蛋白质总量的测定 。

In pH 3.0-4.3 weak acid medium, aluminum-chrome azurol S-Triton X-100 Complex reacts rapidly with proteins to form complexes, which causes the change of absorption spectra, the increase of absorbance near 220 nm and 636 nm, and all ΔA(=A_0-A) values are proportional to the concentration of proteins, Beer's law is obeyed in the range between 0-50 mg/L or 10-80 mg/L for the different proteins. Their molar absorptivities of reactions are 4.23×10~5-2.01×10~6(near 220 nm) and 2.64×10~5-1.64×10~6(near 636 nm) seperately. Based on these, we developed a new spectrophotometric method for the determination of proteins. The method is simple, rapid, and of good selectivity, and has been used for the determination of total amounts of proteins in human serum and urine samples with satisfactory results.