四川晒烟上发现番茄斑萎病毒(TSWV)

Tomato Spotted Wilt Virus (TSWV) on Cigar Tobacco in Sichuan

在近年来的烟草病害调查中,发现四川什邡、绵竹两县的晒烟上一种新病害。田间症状主要是叶面的坏死斑点和环纹及退绿黄化斑,植株矮化,生长不良。电镜下发现大量近圆形的球状粒体,大小为60～80毫微米。病毒分离物在心叶烟和克利夫兰烟上产生退绿环斑,矮牵牛为局部枯斑寄主。分离物的稀释限点为10^(-3)～10^(-4),热钝化温度为45～50℃,体外保毒期为5～6小时。分离物与 TMV、CMV、PVY、PVX 四种抗血清均呈阴性反应。与TSWV 抗血清呈阳性反应。上述结果表明,四川晒烟上的这种病害的病原为番茄斑萎病毒(TSWV)。这是番茄斑萎病毒在我国烟草上的首次报道。

In recent years,in investigation of tobacco diseases in Sichuan a new disease has been found. and the symptoms look like TSWV as discribed on literature.The disease mainly occurred in cigar tobacco areas of Sichuan,with 10% incidence in Shifang and Mianzhu counties. The symptoms of the disease on cigar tobacco are characterized by necrotic rings and spoots on lower leaves of the plant.The necrotic spots and rings are often varied in size and colours.The experiments have been conducted on host range,electron microscopy,serolgical test and physical properties of the isolates 90—176,91—36,91—46,All results have shown that the isolates are TSWV. It is the first report of the TSWV on tobacco in Chian. The author is grateful to Professor Gonsalves in Cornell university,New York State,U.S. A.for his valuable guidance and provision of the serum of TSWV.