单纯检测外周血CD44v6不宜作为筛选肿瘤转移的标志物

Expression of CD44v6 gene in normal human peripheral blood

目的 通过检测CD4 4v6剪接体在正常人外周血的表达情况 ,进而评价其作为肿瘤转移分子标志物的价值。方法 提取 5 0例健康志愿者的外周血单个核细胞 ,应用RT PCR的办法检测CD4 4v6基因的表达 ,并对其产物进行DNA测序。结果 在适当的对照控制条件下 ,5 0例外周血中有 2 9例 (5 8% )CD4 4v6mRNA阳性。结论 在普通人群外周血样本中应用RT PCR的方法CD4 4v6检出率较高 ,因此单纯检测CD4 4v6不适宜作为筛选肿瘤转移的分子标记物。

Objective To investigate if defection of CD44v6 can be used as a marker of cancer progression and metastasis. Methods RNAs were purified from the peripheral blood mononuclear cells of 50 healthy volunteers,subsequently using RT-PCR and DNA sequencing to investigate the expression of CD44v6 gene. Results CD44v6 can be detected from 58% of healthy donors under a proper control.Conclusion Our data showed that CD44v6 gene was expressed by normal peripheral blood nucleated cell in 58% of Peases,suggesting the limitations of CD44v6 for the detection of tumor cells in the blood of cancer patients.