血清IL-2水平对胸水TIL细胞体外扩增及活性的影响

The influence of the level of serum IL-2 on the proliferation rate and anti-tumor activity of the tumor-infiltrating lymphocytes derived from malignant pleural fluid in vitro

目的 通过对血清白介素 2 (interleukine 2 ,IL 2 )水平的检测 ,了解内源性IL 2水平对胸水TIL细胞体外扩增及抗肿瘤活性的影响。方法 对 5 4例有恶性胸水患者 ,第一次抽胸水时无菌收集胸水 ,进行TIL细胞培养 ,并同时进行血清IL 2水平检测。TIL细胞体外杀瘤活性用MTT法检测 ,血清IL 2的水平用ELISA法检测。将IL 2水平较高的 2 7例患者分为 1组 ,IL 2水平较低的 2 7例患者分为另 1组 ,比较两组患者胸水TIL细胞体外增殖速度及杀瘤活性。结果 血清IL 2水平较低组 ,TIL细胞体外培养 8天后扩增 5 9.3± 2 0 .9倍 ,培养 12天后扩增 112 .5± 32 .9倍 ,明显高于IL 2水平较高组的 2 4 .0± 11.0倍和 6 2 .5± 2 1.9倍。IL 2水平较低组TIL细胞体外杀瘤率为 4 5 .3± 9.5 % ,明显高于IL 2水平较高组的 2 6 .7± 7.3% ,P<0 .0 5。结论 血清IL 2水平高低与TIL细胞体外增殖的速度及其杀瘤活性明显相关 ,IL 2水平较低的患者胸水中TIL细胞体外增殖的速度较快 ,杀瘤活性较强。血清IL 2水平可望作为恶性胸水IL 2 /TIL治疗选择的指标。

Objective To explore the influence of serum IL-2 level on the proliferation rate and anti-tumor activity of the tumor-infiltrating lymphocytes (TIL) derived from malignant pleural fluid in vitro. Methods Fifty four patients with malignant pleural fluid were studied. The level of serum IL-2 was measured by ELISA. TILs were purified from malignant pleural fluid of these patients and cultured in the presence of IL-2. MTT assay was used to detect the cytotoxicity against Lovo cell line of TILs in 2 groups (one guroup IL-2>27,amd the other IL-2<27).Results The proliferation rate of TIL in the group with lower level of IL-2 was 59.3±20.9 after 8 days incubation and 112.5±32.9 after 12 days which was higher than those of group with higher IL-2 level. The cytotoxicity against Lovo cell lines at the group with lower level of IL-2 was stronger than that of group with higher IL-2 level( P <0.05).Conclusion The level of serum IL-2 is relate to the proliferation rate and cytotoxicity against Lovo cell line of the TILs derived from the malignant pleural fluid.