摘要
AIM:To detect the origin of hepatocellular carcinoma (HCC)recurring and attempt to propose a new recurrent mechanism.METHODS:Orthotopic liver allotransplantation was performed on male rats with HCC- induced by diethylnitrosamine using female donors.Metastatic tumors in transplanted livers were obtained.A DNA probe that exhibits specificity for the rat Y chromosome was generated by using a set of primers specificto murine srygene.In situ hybridization (ISH) for Y chromosome was used to detected the origin of HCC recurring. Male HCC tissue was designed to be positive control. ISH on female tissue and using non-labeled with DIG probe was thought to be negative control.RESULTS: Positive marks were seen through ISH for Y chromosome in recurrent tumor tissue and positive control.No signal was detected in both negative controls.CONCLUSION: Recurrent HCC after liver transplantation originated from disseminated tumor cells in recipients.Extrahepatic cells homing into liver may be a new HCC recurrence mechanism. Likewise, it implicates that this mechanism is responsible for HCC recurring after hepatectomy.
AIM:To detect the origin of hepatocellular carcinoma (HCC) recurring and attempt to propose a new recurrent mechanism. METHODS:Orthotopic liver allotransplantation was performed on male rats with HCC-induced by diethylnitrosamine using female donors.Metastatic tumors in transplanted livers were obtained.A DNA probe that exhibits specificity for the rat Y chromosome was generated by using a set of primers specific to murine srygene.In situ hybridization (ISH) for Y chromosome was used to detected the origin of HCC recurring.Male HCC tissue was designed to be positive control.ISH on female tissue and using non-labeled with DIG probe was thought to be negative control. RESULTS:Positive marks were seen through ISH for Y chromosome in recurrent tumor tissue and positive control. No signal was detected in both negative controls. CONCLUSION:Recurrent HCC after liver transplantation originated from disseminated tumor cells in recipients. Extrahepatic cells homing into liver may be a new HCC recurrence mechanism.Likewise,it implicates that this mechanism is responsible for HCC recurring after hepatectomy.
基金
Supported by the National Natural Science Foundation of China,No.39870752
