缺锌并补锌对大鼠睾丸及血清睾酮的影响

Effect of Zinc Deficiency and Zinc Replenishment on Testiele and Testosterone in Rats

目的 为了解锌缺乏及补锌对大鼠睾丸的重量及血清睾酮的影响。 方法 将出生后断乳一周的SD大鼠随机分为缺锌组、配喂组、对照组、补锌组和高锌组五组 ,缺锌组、对照组和高锌组分别用缺锌饲料 (锌含量 <1mg/kg)、常锌饲料 (锌含量为 5 0mg/kg)和高锌饲料 (锌含量为 15 0mg/kg)喂养 8周 ,补锌组用缺锌饲料喂养三周后改用高锌饲料喂养五周 ,配喂组用常锌饲料喂养 ,给料量按缺锌组前一天实际进食量添加。 8周后处死 ,用极谱法测定血清中锌含量 ,用原子吸收分光光度法测定睾丸中锌含量 ,采用双抗体放射免疫分析法测定血清中睾酮的含量。 结果 缺锌组大鼠睾丸及血清中锌含量降低 ,睾丸重量减轻。血清中睾酮含量下降 ,缺锌大鼠补锌后可恢复正常。 结论 说明锌缺乏直接影响睾丸及血清中锌的水平 ,从而影响大鼠睾丸的发育及睾酮的分泌与合成。

ObjectiveTo study the effect of zinc deficiency and zinc replenishment on testiele and Testosterone in Rats. Methods SD rats were randomized into zinc-deficient diet (ZD) group, pair-fed (PF) group, zinc normal group (ZN), zinc supplemented (ZS) group and high zinc (HZ) group. ZD group, ZN group, and HZ group were fed up with zinc deficient dietary (content of zinc<1 mg/kg), zinc-normal dietary (50 mg/kg) and high zinc dietary (150 mg/kg) for 8 weeks respectively. ZS group were fed with zinc deficient dietary for 3 weeks, then changed to be treated with high-zincdietary for 5 weeks. PF group were fed with zinc normal dietary according to the actual quantity of ZD group at the previous day. All of the rats were killed after 8 weeks. The concentration of Zn in serum were measured with polarography and with atomic absorption spectroscopy (AAS) in testiele. The concentration of testosterone in serum were measured with RIA. Results The concentration of Zn in serum and testiele were decreased significantly owing to zinc deficiency. The testiele of ZD group grew slowly, the testosterone quantity in serum were lowered but changed to nomal after zinc was supplemented. Conclusions Zinc deficiency decreased zinc concentration in serum and testiele, the same time it affected the development of testiele and synthesis of testosterone.