荧光双标记定量结核杆菌在肺结核诊断中的应用价值

Clinical Value of Hybridization Method in the Diagnosis of Pulmonary Tuberculosis

目的 对痰荧光双标记定量结核杆菌检测法用于肺结核诊断的价值进行评估。方法 135例肺结核患者和 6 0例非肺结核患者的痰液用荧光染色涂片、改良罗氏培养和荧光双标记定量结核杆菌法检测 ,同时测定血结核抗体。结果 菌阳、菌阴肺结核组荧光双标记法的敏感性分别为 83% ,5 3% ,特异性为 10 0 % ;血结核抗体的敏感性分别为 6 3% ,5 3% ,特异性为 95 %。结论 荧光双标记法诊断肺结核的特异性高 ,适合于临床应用 ,而敏感性有待进一步提高。

Objective To evaluate the value of hybridization method in diagnosis of pulmonary tuberculosis.Methods Sputum smear, culture hybridization and blood tuberculosis antibody were studied in 90 cases of smear positive pulmonary tuberculosis, 45 cases of smear negative pulmonary tuberculosis and 60 cases of non-tuberculosis patients. Results The sensitivity of hybridization method in smear positive pulmonary tuberculosis and smear negative pulmonary tuberculosis was 83% and 53%, respectively. The specificity was 100%. While the sensitivity of blood tuberculous antibody was 63% and 53%, the specificity was 95%.Conclusion The high specificity rate renders a positive PCR result, which is clinically useful. Its sensitivity should be improved.