男性不育症血清抗精子抗体水平及其临床意义

Quantitation measurement and meaning of serum antisperm antibody in infertility men

目的探讨抗精子抗体在男性不育患者血清中的定量水平及在诊断治疗中的意义。方法应用酶联免疫吸附试验(ELISA)对120例男性不育患者血清中抗精子抗体总抗体(TIg)、IgG和IgA水平进行了测定,其中30例抗精子抗体抗体阳性患者接受过甲基泼尼松龙治疗。结果120例患者血清中抗精子抗体TIg阳性32例,阳性率26.67%,IgG阳性28例,阳性率23.33%,IgA阳性8例,阳性率6.67%。30例抗精子抗体抗体阳性患者治疗后18例转为阴性,阴转率为60%。结论ELISA方法测定患者血清中抗精子抗体可以作为筛查男性不育原因的首选方法,而且可以评价治疗效果。

Objective To inquire into the quantitative level of antisperm antibody(AsAb)in serum of male sterility and the meaning of diagnosis and curing.Methods Applying enzyme linked immunsorbent assay(ELISA)to measure level of total antibody(TIg)of antisperm antibody in120examples of male sterility's serum;measurement had been in progress to IgG and IgA's standard;among them30examples were positive antisperm antibody,who had accepted the methylprednisone curing.Results The positive example of TIg was32while the positive rate was26.67%in the serum of120male infertilities,28positive examples of AsAb-IgG,positive rate23.33%,and8positive examples of AsAb-IgA,positive rate6.67%;18examples turn to negative by methylprednisone curing in30positiveexamples of anti-sperm antibody,negative conversion rate60%.Conclusion Detecting serum of AsAb by ELISA's method can be regarded as the first methods that screens the reason of the infertility men,furthermore could be used to appraise cure effect.