高脂诱导性肥胖大鼠的胰岛素敏感性与TNF-α及FFAs水平

Changes of fasting TNF-α,FFAs levels and insulin sensitivity in obesity rats induced by high-fat diet

目的观察正常大鼠在高脂饮食诱导下形成肥胖后的胰岛素敏感性及其TNF-α、FFAs的变化。方法9周龄健康雄性Wistar大鼠16只,随机分为正常饲养组(NC熏n=8)和高脂饲养组(HF熏n=8)。喂养10周,比较两组大鼠体重、胰岛素抵抗指数(HOMA-IR)、以及TNF-α、FFAs等的变化。结果经10周高脂喂养,HF组与NC组比较,体重和内脏脂肪组织显著增加(HF组大鼠体重较NC组增加了11.4%,P<0.05;HF组大鼠内脏脂肪组织较NC组增加了20.7%,P<0.01),并出现胰岛素抵抗(HOMA-IR:HF组为8.88±4.25,NC组为3.92±2.26熏P<0.05),空腹FFAs和TNF-α水平显著上升,较NC组分别增加了80.8%(P<0.05)和58.4%(P<0.05),但两组空腹血糖差异无显著性(HF组为7.89±1.46mmol/L,NC组为8.70±1.59mmol/L熏P>0.05)。结论高脂饮食可诱导大鼠肥胖伴胰岛素抵抗,其胰岛素抵抗的形成可能与TNFα、FFAs水平的升高有关。

Objective To observe the changes of fasting FFAs,TNF-αlevels and insulin sensitivity in obesity rats induced by high-fat diet.Method Normal male Wistar rats(9weeks old)were divided into two groups according to taking either normal chow(NC group,3.3Kcal/g,15%-20%calories for fat),or high-fat diet(HF group,5.1Kcal/g,61%calories for fat).Ten weeks later,body weight,visceral fat mass,fasting glucose,fasting insulin,TNF-αand FFAs levels were determined,and HOMA-IR was calculated by the method HOMA-IR=FPG×FINS/22.5.Result Rats of HF group showed significantly body weight and visceral fat mass gain(body weight increased by11.4%in HF group compared with that in NC group,P<0.05;Visceral fat mass increased by20.7%in HF compared with that in NC group,P<0.01)and developed the insulin resistance(HOMA-IR:HF group,8.88±4.25vs NC group,3.92±2.26,P<0.05)after10weeks feeding.The levels of fasting plasma FFAs and TNF-αwere also increased significantly in HF group compared with NC group by80.8%and58.4%(P<0.05).But there were no statistical differences in fasting plasma glucose between the two groups(HF:7.89±1.46mmol/L vs NC:8.70±1.59mmol/L,P>0.05).Conclusion In this study obesity rat with insulin resistance has be induced successfully with high-fat diet,and the state of insulin resistance of obesity rats is correlated with the increased levels of fasting plasma FFAs and TNF-α.