穿琥宁药代动力学及绝对生物利用度的实验研究

Study on pharmacokinetics and bioavailability of Kalil Dehydroandrograpolidi Succinas in plasma of mice

目的研究穿琥宁经小肠给药后在小鼠体内的药代动力学及绝对生物利用度。方法取小鼠60只,随机分为静脉注射组和小肠给药组。小肠给药组小鼠麻醉后,直接在小肠灌注穿琥宁CMC-Na混悬液,静脉注射组为静脉注射穿琥宁溶液,给药后采用HPLC法测定血药浓度。结果经3p97药动学程序处理,穿琥宁经小肠给药的药—时数据符合二室药代动力学模型,其绝对生物利用度为15.88%。结论小鼠经小肠灌注穿琥宁有一定的生物利用度。

Objective To study the pharmacokinetics and bioavailability of kalil dehydroandrograpolidi succinas in plasma of mice.Methods A single dose of kalil dehydroandrograpolidi succinas was i.v or given via instestines to60mices in an open randomized crossover test.HPLC was selected to determine the concentration of kalil dehydroandrograpolidi succinas in plasma.Results The pharmacokinetic parameters of kalil dehydroandrograpolidi succinas was obtained by3p97program and the disposition was conformed to a two-compartment model.Absolute bioavailability of kalil dehydroandrograpolidi succinas was15.88%.Conclusion Kalil dehydroandrograpolidi succinas given via instestines of mice has some degree of bioavailability.