hDaxx融合蛋白高表达降低活化巨噬细胞分泌IL-1β

Over-expression of GFP-hDaxx Fusion Protein Repressed Secretion of IL-1β by Activated Macrophages

目的 研究GFP -hDaxx融合蛋白 (GFP -hDaxx)即时高表达对激活巨噬细胞分泌IL - 1 β的影响。方法 将GFP -hDaxx真核细胞表达载体pEGFP/hDaxx转染巨噬细胞 ,利用Westernblotting检测表达产物。通过hDaxx在巨噬细胞内的即时高表达 ,测定LPS刺激巨噬细胞后 0 .5h、4h、1 2h时 ,活化巨噬细胞分泌细胞因子IL - 1 β的含量。结果 pEGFP/hDaxx在巨噬细胞内成功表达GFP -hDaxx融合蛋白。GFP -hDaxx在巨噬细胞内的高表达 ,使LPS诱导活化的巨噬细胞分泌IL - 1 β量明显降低 (在4h及 1 2h时与对照组差异有显著性 ,P <0 .0 0 1 )。结论 hDaxx即时高表达降低活化的巨噬细胞分泌IL - 1 β。

Objective To detect the effect of over-expressing GFP-hDaxx fusion protein on secretion of IL-1β in activated macrophages. Methods The expression products were detected by using Western blotting in macrophages transfected by pEGFP/hDaxx. IL-1β, which was secreted by macrophages over-expressing immediately GFP-hDaxx fusion protein and activated by LPS, was analyzed at 0.5 h, 4 h,12 h respectively. Results GFP-hDaxx fusion protein immediately over-expressed in macrophages. IL-1β secretion obviously reduced at 4 h and 12 h(P<0.001) after macrophages, which over-expressed GFP-hDaxx, and were activated by LPS. Conclusion Over-expression of GFP-hDaxx fusion protein repressed IL-1β secretion by activated macrophages.