应用滴金法检测孕妇TORCH感染

Clinical study of dot immunological filtration assay detection with TORCH infection in pregnant women

[目的 ]了解大连地区孕妇TORCH各病原体的感染情况及与不良孕史的关系 ,评价滴金法临床应用的可行性。 [方法 ]应用滴金法 ,对 2 4 0例孕妇和 4 6例咨询病人进行了弓形体、风疹病毒、巨细胞病毒、单纯疱疹病毒 4种病原体血清特异性IgM与IgG抗体的检测。IgG抗体的检测同时采用ELISA法对照。 [结果 ]2 4 0位孕妇血清中四种病原体特异性IgM抗体阳性率分别为1 .2 5 %、2 .0 8%、3.75 %、1 .2 5 % ;滴金法检测特异性IgG抗体阳性率分别为 6 .6 7%、87.92 %、79.5 8%、6 0 .4 1 % ;4 6例咨询病人IgM抗体阳性率分别为 6 .5 %、8.7%、1 9.6 %、6 .5 % ;ELISA法的阳性率分别为 6 .2 5 %、86 .2 5 %、77.0 8%、5 9.1 7%。两种方法检测TORCH -IgG阳性率无明显差异 (P >0 .0 5 )。 [结论 ]孕妇TORCH感染的筛选对优生优育是非常有用的指标 ;滴金法为一种灵敏、快速。

In order to know the relation between infection of TORCH on pregnant woman and absorted history in pregnant woman in Dalian .To evaluate the feasibility of DIGFA for the measurement of TORCH . We detected Anti-TORCH IgG and IgM in 240 pregnant woman and 46 querists by using DIGFA . At the same time ,we applied ELISA to detect Anti-TORCH IgG in 240 pregnant woman in order to compare two method . Of 240 pregnant woman ,the antibody IgM positive rate of four pathogent were 1.25%,2.08%,3.75%,1.25%; the antibody IgG positive rate were 6.67%,87.92%, 79.58%, 60.4%. of 46 querists,the antibody IgM positive rate of four pathogens were 6.5% 8.7%19.6% 6.5%; by ELISA, the antibody IgG positive rate were 6.25%,86.25%,77.08%,59.17%.There wasn't significant difference between the antibody IgG positive rate of two methods by statistical analysis ( P> 0.05). [Conclusion ] Detecting the infection of TORCH in pregnant woman is very useful to reveal cause of harmful effects .DIGFA is a sensitive ,rapid and easy measurement