钙调素抗体的亲和层析和酶联免疫分析

Analysis of Calmodulin Antibody by Affinity Chromatography and ELISA

本文利用CaM-Sepharose CL-4B免疫亲和层析柱在乙二醇双(α-氨基乙基醚四乙酸)(EGTA)和酸性条件下,从天然猪脑CaM与甲基化牛血清白蛋白混悬作抗原免疫家兔产生的抗体中分离出两组抗体,并进行了亲和性分析。对文献中有关钙离子的存在可增加钙调素酶联免疫定量测定敏感性的报道进行了研究,并得出不同结果。

Two groups of antibodies raised by immunization to natural porcine-brain Calmodulin (CaM) mixed with MethylatedBovine Serum Albumin were eluted from a CaM- Sepharose CL-4B immunoaffinity chromatography column by EGTA and acidic solution respectively and the assay of their affinity was performed. Our re-consideration comes to an opposite result from the documented conclusion that existence of calcium could increase ELISA sensitivity in CaM quantitative analysis.