施普善对D-半乳糖拟衰老小鼠学习记忆功能的影响

Effect of cerebrolysin on the function of learning and memory of D-galactose induced aging mimetic mice

目的:探讨施普善对D-半乳糖拟衰老小鼠学习记忆能力的影响及其机制。方法:采用D-半乳糖诱导的脑老化小鼠动物模型,分别用游泳水迷宫测定小鼠学习记忆能力;用化学发光法测定超氧化物歧化酶(SOD)活性;用硫代巴比妥酸比色法测定丙二醛含量。结果:D-半乳糖模型组小鼠的游泳时间明显延长犤(44.91±12.86)s犦、正确次数明显降低犤(2.58±1.87)次/d犦,与正常对照组犤(28.11±12.75)s,(4.33±1.72)次/d犦相比,差异有显著性意义(t=3.694,t=3.258,P<0.01)。应用施普善6周后,其大、中剂量组小鼠的游泳时间明显降低犤(28.06±13.06),(27.85±13.21)s犦,正确次数明显增加(4.58±1.66),(4.63±1.81)次/d犦,与D-半乳糖组相比,差异均有显著性意义(t=3.574,3.508;t=3.416,3.349,P<0.01);并能显著提高脑组织中SOD的活性犤(30232.38±5133.53),(29653.26±4878.64)nkat犦,降低丙二醛含量犤(630.00±59.40),(626.74±49.68)nmol/g犦,与D-半乳糖组犤(22892.74±4540.07)nkat,(749.69±92.80)nmol/g犦相比,差异均有显著性意义(t=3.418,3.277;t=3.168,3.337,P<0.01)。结论:施普善能明显的改善D-半乳糖诱导的脑老化小鼠学习记忆能力,提高脑组织中SOD的活性,降低丙二醛的含量。

AIM:To investigate the influence and mechanism of cerebrolysin on the learning and memory of D galactose induced aging mimetic mice. METHODS:The memory function was tested in Morris water maze in the aging mimetic models of mice induced by D galactose.Superoxide dismutase(SOD) activity was measured with chemiluminescence assay. Malondialdehyde(MDA) content was measured with thiobarbituric acid chromatometry. RESULTS: In the D galactose model mice, the swimming time[(44.91±12.86) s]was prolonged significantly,and the correct number was decreased significantly[(2.58±1.87) times per day],as compared with the normal group [(28.11±12.75) s,(4.33±1.72) times per day](t=3.694,t=3.258,P< 0.01).Six weeks after the administration of cerebrolysin, the swimming time was significantly shortened in the middle and large dosage group[(28.06±13.06) and(27.85±13.21) s,respectively],and the correct number was increased significantly[(4.58±1.66) and(4.63±1.81) times per day,respectively],as compared with the model group(t=3.574,3.508;t=3.416,3.349,P< 0.01).Significant increase in the activities of SOD [(30 232.38±5 133.53) and(29 653.26±4 878.64) kat, respectively]and significant decrease in the contents of MDA[(630.00±59.40) and(626.74±49.68) nmol/g,respectively]were found in the middle and large dosage group of cerebrolysin in comparison with the model group[(22 892.74±4 540.07) kat and(749.69±92.80) nmol/g,respectively](t=3.418,3. 277;t=3.168,3.337,P< 0.01). CONCLUSION:Cerebrolysin can remarkably improve the learning and memory function of D galactose induced aging mice,enhance SOD activity and decrease MDA content in brain tissue.