创伤后应激障碍大鼠动物模型行为学表现及学习记忆功能的变化

Changes of behavior and function of learning and memory of post-traumatic stress disorder model of rat

目的:制备创伤后应激障碍(post-traumaticstressdisorder,PTSD)大鼠模型并观察其行为学表现和学习记忆功能的变化。方法:利用幽闭+电击方式制备Wistar大鼠PTSD实验动物模型,观测其总体运动水平和前25min各运动类型成分的变化,以及利用穿梭箱实验检测PTSD大鼠学习记忆功能的改变。结果:PTSD大鼠总体运动水平发生两极性变化,多数(7/9)降低,少数(2/9)升高,与对照组比较均差异均有显著性意义(t=10.230,P<0.01);前25min各运动类型成分中,静止状态和逃避状态显著增加,对照组和PTSD组分别为(11.08±1.67)%和(24.24±9.69)%(t=-6.878,P<0.05),梳理状态成分显著减少,对照组和PTSD组分别为(28.34±6.86)%和(13.13±7.02)%(t=2.234,P<0.05);PTSD大鼠学习记忆功能检测发现,主动回避反应显著下降(P<0.01),回避反应失败显著增加(P<0.01)。结论:用幽闭+电刺激制备大鼠PTSD实验动物模型,行为学出现明显异常,学习记忆功能受损,与临床表现有较好的拟似性,重复性高。

AIM:To develop an experimental rat model of post traumatic stress disorder(PTSD) so as to detect its behavior and function of learning and memory. METHODS:The rat model of PTSD was made by means of imprisonment and electric shock.The total behavior level of PTSD rats and the composition of motor types were detected within the previous 25 minutes.The function of learning and memory was detected by shuttle box test. RESULTS:The total behavior level of PTSD rats presented polarization,which decreased in the most of rats(7/9) and increased in a few(2/9). There was a significant difference between the PTSD rats and normal rats(t=10.230,P< 0.01).Within the previous 25 minutes, the percentage of stillness and escaping was increased to(11.08±1.67)%for the control group and(24.24±9.69)%for the PTSD group(t=-6.878,P< 0.05),the percentage of grooming was significantly decreased to(28.34±6.86)%and(13.13±7.02)%for the two groups respectively(t=2.234. P< 0.05). In the test of learning and memory function, active response to avoidance significantly decreased(P< 0.01),and failed response to avoidance significantly increased(P< 0.01). CONCLUSION:The PTSD rat model produced by imprisonment and electric shock has remarkable abnormal behavior and impairment of learning and memory.It is similar to the clinical symptoms.