针刺对帕金森病模型大鼠血液T淋巴细胞亚群的影响

Effect of acupuncture on blood T lymphocyte subpopulation in rat model with Parkinson's disease

目的:探讨针刺治疗对帕金森模型大鼠T淋巴细胞亚群的影响。方法:采用脑内黑质部注射6-羟基多巴制备帕金森病大鼠模型,分别采用针刺、美多巴及针刺结合美多巴对模型大鼠进行治疗。采用免疫荧光法测定T细胞亚群数。结果:CD3,CD4,CD8在正常对照组为(68.7±8.8)%,(42.1±8.0)%,(31.4±6.4)%;模型自然恢复组为(54.9±8.4)%,(31.9±7.8)%,(23.9±4.2)%;针刺治疗组为(66.8±8.7)%,(32.1±9.9)%,(29.9±6.6)%;美多巴治疗组为(68.4±7.3)%,(31.9±5.4)%,(31.2±7.6)%;针刺结合美多巴治疗组为(69.3±7.6)%,(32.8±8.0)%,(32.3±6.3)%。与正常对照组比较,模型自然恢复组CD3,CD4,CD8均明显降低(t=1.814～1.831,P<0.05)。与模型自然恢复组比较,针刺、美多巴及针刺结合美多巴治疗后CD3,CD8的百分数明显升高(t=1.813～1.830,P<0.05)。结论:针刺治疗帕金森病模型大鼠有效可能与调节T淋巴细胞亚群的比例及改善其功能有关。

AIM:To investigate the effect of acupuncture on blood T lymphocyte subgpopulation in rat model with Parkinson's disease(PD). METHODS:PD models of rats were established by injecting 6 OHDA into substantia nigra.Acupuncture,medopa,and combination of acupuncture and medopa were used to treat the model rats respectively.The amount of T lymphocyte subpopulations was determined with immunofluorescence method. RESULTS:The percentages of CD3, CD4 and CD8 were(68.7±8.8)%, (42.1±8.0)%and(31.4±6.4)%for the control group,(54.9±8.4)%,(31.9±7.8)%and(23.9±4.2)%for the model group, (66.8±8.7)%,(32.1±9.9)%and(29.9±6.6)%for the acupuncture group,(68.4±7.3)%,(31.9±5.4)%and(31.2±7.6)%for the medopa group,and(69.3±7.6)%,(32.8±8.0)%and(32.3±6.3) for the therapeutic alliance group.Compared with the control group, the percentages of CD3, CD4,CD8 were obviously decreased in the model group(t=1.814-1.831,P< 0.05).Compared with the model group, the percentages of CD3,CD8 were significantly increased in the three treatment groups (t=1.813-1.830, P< 0.05). CONCLUSION:Curative effect of acupuncture in the treatment of PD may be related with the regulation of the ratio of T lymphocytes' subgroups and its functional improvement.