10种郁李仁有效成分的分析鉴定研究

Study on the Analysis of Yuliren by TLC and Electrophoresis

利用薄层扫描法测定了10种郁李仁的苦杏仁甙含量。以欧李、毛叶欧李、榆叶梅、李种子中含量高于2.25%。郁李仁甙A和郁李仁甙B仅存在于欧李、毛叶欧李、郁李、长梗郁李、麦李等5种植物种子中。郁李仁甙A在麦李的含量为0.29%,其它4种含量差异不大,均大于0.70%。对10种郁李仁可溶性蛋白进行了SDS-Tris-甘氨酸聚丙烯酰胺凝胶电泳分析,表明除欧李、毛叶欧李、郁李、长梗郁李、麦李具有相似的谱带外,其它种郁李仁电泳色谱间有一定的差异。

Yuliren, a commonly used Chinese traditional drug, has a purgative and diuretic activity. Thin-layer chromatographic separation and determination of the main components in Yuliren were carried out. The results showed that amygdalin exists in 10 Prunus species of Yuliren and the contents of Prunus humilis, P. dictyoneura, P. triloba, P. salicina were over 2.25%. Prunuside A and prunuside B only exist in seeds of P. humilis, P. dictyoneura, P. japonica, P. japonica var. nakaii and P. glandulosa. This method was simple and effective. The water-soluble seed proteins of 10 Prunus species were studied by electrophoresis on SDS-polyacrylamide gel. Their electrophoretograms revealed some relation and differences among them. P. humilis, P. dictyoneura, P. japonica, P. japonica var. nakaii and P. glandulosa showed to be most closely related, while the other species could be distinguished from each other according to the band number and the electrophoretic pattern of water-soluble protein.