尼莫地平缩小脑梗死小鼠梗死体积与时间窗的关系

Relationship of nimodipine for reducing infarct volume with therapeutic time window in cerebral ischemic mice

目的:研究尼莫地平缩小脑梗死小鼠梗死体积的最佳时机。方法:经右侧颈总动脉将尼龙单丝线栓至大脑中动脉造成永久性缺血模型。经尾静脉及腹腔给药。动物均于术后第7天处死取脑,用TTC染色、图像分析仪测定脑梗死体积。结果:单缺组脑梗死体积最大,为(224.00±56.88)mm3,术后6h以内为(110.23±43.29)～(129.64±65.42)mm3,术前给药组为(107.12±62.34)mm3,脑梗死体积较小,术后12h以后给药组为(173.82±57.28)～(242.67±65.28)mm3,脑梗死体积介于二者之间。结论:术前及术后6h以内给药可使小鼠脑梗死体积减小,这一段时间可能为治疗时间窗。

AIM:To find out the optimal opportunity of nimodipine in the reduction of infarct volume in the mice of focal cerebral ischemia. METHODS:Focal ischemic models of middle cerebral artery occlusion(MCAO) were a stablished by inserting nylon monofilament through the right common carotid artery.Nimodipine was injected through caudal vein and abdominal cavity.Seven days after the operation, the animals were killed and the brains were taken out.The infarct size was measured by TTC staining and imaging analyzer. RESULTS:The infarct volume was the biggest in the simple ischemia group[(224.00±56.88)mm3],and the smallest in the preoperative treatment [(107.12±62.34)mm3].Within 6 hours after the operation, the infarct size was(110.23±43.29)to(129.64±65.42)mm3 in the simple ischemia group.In the postoperative treatment group, the infarct size was(173.82±57.28)to(242.67±65.28)mm3 12 hours after the operation. CONCLUSION:The optimal therapeutic time window of nimotop was before or less than 6h after MCAO,during which,the infarct size was reduced.