中潜伏期听觉电反应对糖尿病大鼠中枢神经病变的评估作用(英文)

Evaluating role of auditory middle latency response in diabetic central neuropathy of rats

背景:糖尿病能引起中枢神经病变,中潜伏期听觉电反应(auditorymiddlelatencyresponse,MLR)在糖尿病时的动态变化研究至今尚不十分清楚。目的:研究MLR在糖尿病中的变化,探讨MLR在早期诊断糖尿病中枢神经病变中的作用。设计:用完全随机法,采用实验对照方式,进行纵向观察研究。地点和材料:在暨南大学医学院生理研究室进行实验。用24只实验性糖尿病大鼠进行研究。方法:应用链脲佐菌素腹腔注射法,建立实验性糖尿病动物模型。在成模前后按时间顺序动态观测血糖、MLR的变化。主要观察指标:MLR的波峰潜伏期(peaklatency,PL)、波峰间潜伏期(interpeaklatency,IPL)。结果:建模后4周实验性糖尿病组MLR的PL(ms)各波延长犤P0(7.19±0.97)、Na(11.90±1.53)、Pa(18.77±1.45)、Nb(32.86±3.12)、Pb(50.32±1.68)犦(P<0.05)。6周后出现MLR各波PL、Pa-Pb的IPL犤(31.10±1.59)ms犦延长(P<0.01)。8周后MLR各波PL、P0-Pa犤(13.57±1.71)ms犦,Pa-Pb犤(35.17±2.81)ms犦IPL均延长(P<0.05或P<0.01)。糖尿病组血糖与MLR的PL,IPL各波变化之间无相关,糖尿病组病程与MLRNa,Nb,Pb波PL变化呈正相关。结论:糖尿病早期MLR出现PL和IPL延长,该变化与病程进展有相关关系,MLR是早期诊断糖尿病中枢神经病变的客观灵敏指标。

BACKGROUND:Diabetes can induce diabetic central neuropathy(DCN)The dynamic changes of auditory middle latency response(MLR) in diabetes mellitus have been not clear as yet. OBJECTIVE:To study the dynamic changes in MLR of diabetic rats and investigate the role of MLR in the early diagnosis of DCN. DESIGN:Completely randomized controlled trial conducted between experimental group and control group. SETTING and MATERIALS:A total of 24 experimental diabetes(ED) rats model were used in our laboratory. METHODS:Before and after 2,4,6,8 and 10 weeks of ED modeling, plasma glucose and MLR were measured. MAIN OUTCOME MEASURES:The peak latencies(PL) and interpeak latencies(IPL) of MLR. RESULTS:The PL(ms) of P0(7.19±0.97),Na(11.90±1.53),Pa(18.77±1.45),Nb(32.86±3.12),Pb(50.32±1.68) of MLR were longer after 4 weeks of ED modeling in ED group as compared with those of the normal control group(t=2.524,P< 0.05).All PL and Pa-Pb[(31.10±1.59)ms]IPL of MLR were prolonged after 6 weeks in ED group(t=2.891-3.474,P< 0.01).All the PL,P0-Pa[(13.57±1.71)ms],Pa-Pb[(35.17±2.81)ms] IPL of MLR were prolonged after 8 weeks in ED group(t=2.512,P< 0.05;t=2.891-3.271,P< 0.01).There was positive correlation between the duration of diabetes and Na,Nb,Pb,PL of MLR. CONCLUSION:The PL and IPL of MLR were prolonged in early ED.MLR may serve as an objective and sensitive index in the early diagnosis of DCN.