槐耳菌丝体多糖的研究(Ⅱ)——槐耳多糖的组成及摩尔比测定

Studies on the Constituents of Polysaccharide from the Hyphae of Trametes Robiniophila(Ⅱ)——Identification of Polysaccharide from the Hyphae of Trametes Robiniophila and Determination of Its Molar Ratio

将水解后的多糖转化成还原乙酰化衍生物,用气相层析法检定了槐耳多糖由L-岩藻糖、L-阿拉伯糖、D-木糖、D-甘露糖、D-半乳糖、D-葡萄糖组成,纸层析未检出岩藻糖。采用OV-225固定液的填充柱,对以上6种单糖还原乙酰化衍生物的分离鉴定获得满意结果,并在该柱上测定了槐耳多糖组成中单糖的摩尔比。

After conversion of the hydrolyzed polysaccharride from the hyphae of Trametes robiniophila Murr. into alditol acetate derivetives the components of PS-T was identified by GC. PS-T is composed of Lfueose, L-arabinose, D-xylose, D-galactose, D-mannose and D-glucose, The molar ratio of these sugars were also determined. The column, packed with 3% OV-225 on AW-DMCS Chromosorb W (80/100mesh) is used satisfactorily for separating and identifying alditol acetate derivetives of these six sugars(L-fueose, L-arabinose, D-xylose, D-galaetose, D-mennose, D-glucose).