葡萄球菌DNA的原生质体转化

Ptotoplast Transformation of Staphylococcal DNA

用三株金黄色葡萄球菌多剂抗生素耐药菌株80022,82022和82062的质粒DNA和总DMA,分别对受体菌金黄色葡萄球菌RN1304(Y-1)的原生质体进行转化试验。平衡期初期的受体菌用溶菌酶和溶葡萄球菌素处理,所获得的原生质体与质粒DNA混合,以PEG诱导,涂布于含药的双层DM_3再生培养基上,即可获得Tc^r或Cm^r转化子。转化率为10~2—10~3转化子/μg DNA。

Experiment on protoplast-transformation of S. aureus RN1304(Y-1) by plasmid DNA or total DNA extracted from multiple antibiotic resistant staphylococcal strains (80022,82022 and 82062) was reported. Protoplasts were prepared by treating recipients at early stationary phase with lysozyme and lysostaphin. Mixed protoplasts and DNA, then treated with polyethylene glycol (molecular weight. 6000), Tc^r or Cm^r transformants were obtained. The frequencies of protoplast transformation were 10~2—10~3 transformants per μg DNA.