摘要
目的 以猪为实验动物来探索哺乳动物出生后短时期内肝脏抗氧化功能的发育特点。方法 选择新生仔猪 15头 ,分别于出生当日 ( 0d)、出生后 3d及 7d屠宰取样 ,测定肝脏中 3种主要抗氧化酶的活性及脂质过氧化产物和羟自由基的含量。结果 新生仔猪肝脏中 3种抗氧化酶活性的变化趋势不同。超氧化物歧化酶 (SOD)总活性、含Cu、Zn和Mn的SOD的活性均在刚出生时较高 ,以后随着日龄的增加呈下降趋势。谷胱甘肽过氧化物酶活性在 0d～ 3d之间下降 (P <0 .0 5 ) ,4d以后回升 (P <0 .0 5 )。过氧化氢酶活性在生后 3d内升高 (P <0 .0 1) ,以后保持稳定。仔猪刚出生时 ,肝脏中羟自由基、脂质过氧化物和丙二醛的浓度都比较高 ,以后减少。结论 动物刚出生时肝脏中自由基浓度较高 ,肝组织的脂质过氧化反应严重。出生后短期内 ,肝脏的抗氧化酶活性及抗氧化状态都出现了明显变化。
Objective To study the developmental features of antioxidation capability of the liver of neonates. Methods Fifteen neonatal pigs were sacrified and livers were sampled on the day of birth, day 3 and day 7 after birth, respectively. The activities of antioxidative enzymes, the content of lipid peroxides and hydroxyl radicals were measured. Results The activities of superoxide dismutase (SOD), CuZnSOD and MnSOD were the highest in liver at birth, and decreased gradually in the first postnatal week. The activity of glutathione peroxidase was lowest on day 3 (P<0.05). In contrast, the activity of catalase increased from newborn to day 3 (P<0.01) and maintained at a steady level between day 4 and day 7 after birth. Contents of hydroxyl radicals, lipid peroxide and malondialdehyde were considerably high in the liver of newborn piglets. Conclusion There were higher radical concentrations and significant lipid peroxidation in the liver of animals at birth. Rapid changes of antioxidative status of liver developed during the short postnatal period.
出处
《肝脏》
2004年第1期28-30,共3页
Chinese Hepatology
