FAS抗原与慢性丙型肝炎关系的研究

Study of relationsip between FAS antigen expression and HCV RNA level in patients with chronic hepatitis C

目的 通过观察慢性丙型肝炎 (CHC)患者肝组织及外周血单核细胞 (PBMC)FAS抗原表达对CHC病情的影响 ,进而探讨FAS系统及HCVRNA水平与CHC的关系及临床意义。方法 采用肝活检组织病理、免疫组织化学、PCR及血液生化等方法检测 41例CHC患者的肝组织和PBMC中FAS抗原、血清HCVRNA及肝功能 ,观察肝组织和PBMC中FAS抗原表达情况与血清HCVRNA水平及肝损伤的关系。结果 ( 1)CHC患者PBMC的FAS阳性率为41.5 % ( 17/ 41) ,肝组织FAS阳性率为 65 .6% ( 2 1/ 3 2 )。 ( 2 )CHC患者PBMCFAS阳性组血清HCVRNA和ALT水平均显著高于FAS阴性组 (P <0 .0 5 )。结论 ( 1)CHC患者肝组织和PBMC中FAS表达与肝组织炎症及血清病毒载量有关 ;( 2 )FAS系统可作为观察CHC病情的新指标 ,协助诊断与治疗。

Objective To study the expression of FAS antigen in peripheral blood mononuclear cells(PBMC), in the liver tissue and the level of HCV RNA in serum of patients with chronic hepatitis C(CHC), and their relation to liver damage. Methods FAS antigen in PBMC and in the liver of patients with CHC were detected by immuno-histochemistry and serum HCV RNA were detected by PCR. Liver tissue section and serum ALT were detected simultaneously. Results (1) The rate of FAS positive in PBMC of patients with CHC was 41.5%(17/41) in the liver tissue was 65.6%(21/32). (2) Both serum HCV RNA and ALT level were higher significatly in patients of PBMC FAS(+) than those of PBMC FAS(-).(P<0.05). Conclusion (1) The expression of FAS antigens in PBMC and in the liver tissue are correlated to liver inflammation and serum HCV RNA in patients with CHC. (2) FAS system may play a new assistent role on dagnosis and treatment of CHC.