膜磷脂分离测定缺血再灌时大鼠心肌膜磷脂的变化

Quantitative analysis of membrane phospholipids by TLC in ischemic - reperfused rat heart

使用薄层层析(TLC)技术分离磷脂(磷脂酰胆碱、磷脂酰乙醇胺)和血小板活化因子,定磷法测定膜磷脂含量。其基本过程为:组织样本经Bligh—dyer脂质提取、TLC技术分离各磷脂组分,再用定磷法测定磷脂的含量。结果发现:急性心肌缺血/再灌注可引起大鼠在体心肌膜磷脂的降解,心肌总磷脂、磷脂酰胆碱、磷脂酰乙醇胺分别降低38.7％,46.1％,42.2％,提示膜磷脂在急性心肌缺血/再灌注中具有重要作用。

Thin-Layer Chromatography (TLC)used for separation and quantitation of phospholipids including phosphatidyl-choline (PC), Phosphatidyl-ethanolamine (PE), platelet activating activating-factor (PAF) were studied. The experimental processes were: lipid extract from tissue sample by Bligh-dyer methods, separation of phospholipids by TLC. and quantitation of phospholipids by phosphorus quantitative method. Using this method had found that reperfusion caused membrane phospholipids degradation in rat heart in situ subjected to 5 min of ischemia and followed by 10 min of reperfusion, myocardial total phospholipid, PC, PE decreased by 38.7, 46. 2 and 42.2%. respectively. Results indicated that membrane phospholipid play an important role in the genesis of ischemic-reperfusion induced myocardial damage.