抗瘤酮A_(10)的药效学实验研究

The studies of the pharmacodynamics of antineoplaston A_(10)

本文观察了不同剂量的抗瘤酮A_(10)对动物移植性肿瘤小鼠S_(1(?)0)、大鼠W_(25(?))、裸小鼠荷人乳腺癌细胞(Bca—P6)和人胃癌细胞(MGC)的抑制效应。A_(10)大剂量组(4000mg·kg^(-1))对小鼠S_(1(?)0)的抑制率为32.5％(P<0.05);对裸鼠体内的Bca—P6和MGC细胞的抑制率分别为54.4％(P<0.001和33.9％(P<0.05);2000mg·kg^(-1)剂量对W_(206)的抑制率为41.8％(P<0.05);各中、小剂量对上述瘤株均没有明显的抑制作用.体外试验结果表明,A_(10)大剂量组(终浓度为0.12mol·L^(-1))与Bca—P6细胞接触4h可达到一个对数杀伤(杀伤90％的细胞),24h可达到2个对数杀伤(杀伤99％的细胞);HL—60细胞与药液接触24h后洗去药液继续培养48h和72h,可达到一个对效杀伤,96h可达到2个对数杀伤。其余各剂量组杀伤效果不明显。

This paper describes the inhibitory effects of antineoplaston A10 with using different dosages against the mouse S180, rats W256 and Bca - P6 and MGC cell (human) in nude mouse. When the administered dosage of oral formulation antineoplaston A10 was 4000mg · kg-1 · 24h-1 against the S180 for 10d . inhibitory rate was 32. 5%( P <0. 05). A-gainst the Bca -P6 and MGC cell in nude mouse for 15 and 16d. the inhibitory rate were 51. 4% ( P <0. 001) and 33. 9%( P <0. 05) respectively. 2000 mg · kg-1. 24h-1 against the W256 for 10d. the in- hibitory rate was 11. 8% ( P <0. 05). The results rsing relative clone - survival test in vitro showed that when the terminal concentration of 0. 12mol · L-1 of antineoplaston A10 reacted with Bca - P6 cell for 4h, 1LK was reached. For 24h. 2LK was obtained. Using cell count test, HL - 60 cell reacted with antineoplaston A10 for 24h and were cultured continually to 48h and 72h after washed drug, 1LK was reached. To 96h, 2LK was obtained.