盐酸小檗碱胺对乙酰胆碱引起的内皮依赖性血管舒张的影响

The influence of hydrocholoride berbamine to the indothelium derived blood vessel relaxation induced by Acetylcholine

在无Ca^(2+)krebs液中,乙酰胆碱(Ach)诱发兔主动脉舒张受抑制.恢复ca^(2+)浓度后,抑制解除。在去甲肾上腺素(NE)04μm ol·L^(-1)组中,盐酸小檗胺(HBA)I.10μmol·L^(-1)和维拉帕米(Ver)0.1.1μmol·L^(-1)对Ach的舒张反应无抑制,且HBA还有促进舒张的趋势.而HBA100μmol·L^(-1)和Ver10μmol·L^(-1)则呈明显抑制作用。在KCI20mmol·L^(-1)组中,HBA,Ver3个浓度都对Ach舒张有促进作用。结果提示,HBA对内皮依赖性舒张的影响是复杂的,较高浓度抑制舒张.较低浓度促进舒张。可见,HBA对内皮细胞Ca^(2+)转运的影响.也即对EDRF的影响是复杂的。

In the Ca - free solution .the relaxation induced by acctylcholine ( Ach ) in the rabbit aortic strips is inhibited and the inhibition is removed when the concentration of the Ca2+ is restored. In the groupe of ME ( norepinephrine ) 0.4 μmol · L-1 hydro-choloride berbamine (HBA ) 1, 10μmol · L-1 and Verapamie (Ver) 0. 1, 1μmol · L-1 did not inhibit the relaxation induced by Ach,and HBA has tendency of promoting the relaxation. While HBA 100μmol · L-1 and Ver 10μmol · L-1 inhibit the relaxation obviously. In the group of KCl,the three concentration of HBA and Ver all have the tendency of promoting the relaxation. The result suggest: that the effection of HBA to the relaxation of in-dothelium dependence is complicated, the higher concentration inhibit the relaxation and the lower promote it. It prove that the effection of HBA to the transportation of indothelium's Ca2+ or to the release of the EDRF is complicated