黄皮酰胺抗脂质过氧化及对氧自由基清除作用

Anti-lipid peroxidation and oxygen free radical scavenging activity of clausenamide

黄皮酰胺是一种生物碱,可抑制铁-半胱氨酸体系引起的大鼠脑,心,肝和睾丸微粒体脂质过氧化,电子自旋共振法研究表明,黄皮酰可清除由佛波醇豆蔻酸乙酸酸酯(PMA)刺激人多形核白细胞(PML)所产生的氧自由基,自旋探针测氧法结果表明,对PMA刺激PML时的氧消耗无影响,在Fenton反应体系中,对羟自由基的清除率为36.6％,在黄嘌呤-黄嘌呤氧化酶和光照核黄素体系中,对超氧阴离子的清除作用分别为21.2％和16.2％。结果提示黄皮酰胺对氧自由基的直接捕捉作用是其抗脂质过氧化作用的机理之一。

Clausenamide (Clau) is an alkaloid. The present investigation demonstrated that Clau inhibited ferrous-cysteine induced lipid peroxidation (malondialdehyde formation) of microsomes from the rat brain, heart, liver and testes. Electron spin resonance (ESR) detected that Clau scavenges oxygen radicals produced by human polymorphonuclear leukocytes (PML) stimulated by phorbol myristate acetate (PMA). However, no inhibitory effect of Clau on oxygen consumption of PML during respiratory burst was measured by spin label oximetry. In Fenton reaction system, 36.6% of OH was scavenged by Clau. It scavenged Oi by 21.2% and 16.2%, in xanthine-xanthine oxidase and UV-irradiation of riboflavin systems, respectively. It may be concluded that Clau exhibits an antioxidant activity via scavenging oxygen free radicals.