三硝基甲苯血红蛋白加合物作为生物监测的标志物

Hemoglobin adducts of trinitrotoluene as a biomonitoring marker

在前文已证实TNT进入体内可与蛋白共价结合的基础上,用[^(14)C]TNT法比较了TNT与血红蛋白、血浆蛋白及肝、肾组织蛋白四种蛋白加合物的共价结合,从加合物的剂量反应关系,在体内存留时间以及取样方便等条件来看,血红蛋白加合物是生物监测最有希望的指标,文中对它作为生物监测的意义进行了讨论。

For years there is a great need in the development of a sensitive bio-monitoring method for occupational protection in China. Since the covalent-binding of TNT to proteins have been proved in the previous study, the main objective of this paper is to explore the possibility of using the adducts as a marker for bio-monitoring TNT exposure.When a single dose of 1, 10, or 50 mg ·kg-1 of [14C]TNT was administered to a rat, covalently bound radioactivity could be measured in the hemoglobin, plasma protein as well as proteins in the liver and kid-ney. Within 4 h the covalent binding was dose-dependent, particularly in the low dose groups. The bound radioactivity at 10 mg · kg-1 was about 10 times of that at 1 mg·kg-1.A comparison between the levels of hemoglobin and plasma protein adducts from 1 h-8 d after dosing showed that unlike plasma protein the concentrations of hemoglobin adducts were more stable as reflected by a much slower decline within 8 d. Therefore, TNT-hemoglobin adduct appeared to be a good marker for bio-monitoring TNT exposure.