猪胆酸钠对人早幼粒白血病细胞系(HL-60)作用机理的初步探讨

Preliminary study on molecular mechanism of action of swine bile acid-sodium salt on the cells in human promyelocytic leukemia cell line HL-60

观察了猪胆酸钠和维甲酸对人早幼粒白血病细胞系(HL-60)体外诱导分化过程中细胞膜脂肪酸的组成、细胞蛋白激酶C(PKC)活性及c-myc癌基因表达的变化,初步探讨猪胆酸钠对HL-60细胞作用的分子机理,结果表明,经100～400μg·ml^(-1)猪胆酸钠处理3d的HL-60细胞,其PKC活性和c-myc表达与对照组比较均明显下调;1μmol·L^(-1)维甲酸诱导3 d的细胞,PKC活性未见明显变化,而癌基因c-myc表达则降至对照组的17％;无论是猪胆酸钠(400 μg·L^(-1))还是维甲酸(1μmol·L^(-1))诱导3d的HL-60细胞,其细胞膜脂肪酸组成均无明显改变。

The membrane fatty acid composition, protein kinase C (PKC) and c-myc expression in the differentiation of human promyelocytic leukemia cell line HL-60 induced by swine bile acid-sodium salt (SBA Na) or retinoic acid (RA) in vitro were measured to investigate the molecular mechanism of the effect of SBA-Na on HL-60 cells. The down-regulation of both PKC activity and c-myc expression in HL-60 cells treated by 100-400 μg·?ml-1 SBA-Na for 3 d was observed, whereas a great decrease of c-myc expression but lack of obvious change for PKC activity in HL-60 cells induced by 1 μmol ·L-1 RA for 3 d was monitored, as compared with the control HL-60cells. No any involvement of membrane fatty acid composition was determined in the HL-60 cells induced by 1 μmol·L-1 RA or 400 μg·ml-1 SBA-Na for 3 d. The experimental results indicate that the biological activity of SBA-Na to induce the differentiation of HL-60 cells appears to be related to the potential of the agent to inhibit PKC and c-myc expression in the cells.