胸腺因子D对老年大鼠肝微粒体,线粒体钙离子转运的影响

Effects of thymic factor D on Ca^(2+) transfer of liver microsome and mitochondria in aged rats

应用^(45)Ca研究了胸腺因子D(TFD)对老年大鼠肝脏亚细胞结构Ca^(2+)转运的影响,结果表明,与成年对照鼠(7月龄)相比,雄性老年大鼠(24月龄)肝微粒体,线枉体膜Ca(2+)主动摄取能力降低,肝微粒体Ca(2+)被动释放增多,给老年大鼠TFD 2 mg·kg^(-1)sc隔日一次共3月,可使其肝微枉体Ca(2+)主动摄取能力提高,Ca(2+)被动释放减少,有利于维持细胞内钙穗态,同时TFD还可逆转老年大鼠肝微粒体,线粒体膜流动性的降低及过氧化脂质的增高。

The experiments by using 45Ca were performed to investigate Ca2+ transfer of the liver subcellular fraction in the male aged Wistar rats (24-month-old) and the effects of thymic factor D (TFD), extracted from the pig thymus, on these parameters. It was found in the aged rats that Ca2+ uptake of the liver microsome and mitochondria showed a significant decrease, with a simultaneous increase inthe passive Ca2+ release from the liver microsome. TFD, given to the aged rats, at a dose of 2 mg·kg-1 sc every other day for 3 month increased Ca2+ uptake of the liver microsome and decreased the passive Ca2+ release from the liver microsome, showing a beneficial effect on maintaining intracellular calcium homeostasis. In addition, TFD reversed an increase of lipid peroxides and a decrease of membrane fluidity in the liver microsome and mitochondria of the aged rats