反相高效液相色谱法测定人血浆中盐酸维拉帕米的研究

Determination of verapamil in human plasma by reversed-phase HPLC

应用乙醇-水-二乙胺-冰醋酸(250∶300∶0.125∶0.25)为流动相,在反相 YWG-C_(18)H_(37)柱上,以安定(diazepam)为内标,对盐酸维拉帕米建立了适合于临床药理和临床化学应用的 HPLC测定法。本法简便、快速、灵敏,重现性好;流动相对人体无毒并可循环使用。样品用乙醚-二氯甲烷(10∶8)提取,盐酸维拉帕米及安定的平均回收率大于95%,CV_(日内)<1.8%,CV_(日间)<4.6%。

A liquid chromatographic method was deve-loped for the determination of verapamil in humanplasma using a reversed-phase column(YWG-C_(18)H_(37))with diazepam as interhal standard and ethylalcohol-water-diethyl amine-glacial acetic acid(250:300:0.125:0.25)as mobile phase used inrecycle mode.The absolute recoveries of verapamiland internal standard were found to be above 95%and the method was linear form 50～125ng with acorrelation coefficient of 0.9995.The coefficientof variation was less than 1.9% for within day(n=7)and less than 4.1% for between day(n=8)respectively.