期刊文献+

实时PCR在肿瘤诊断中的应用

Real-time PCR technology for tumor diagnosis
下载PDF
导出
摘要 以荧光能量传递技术为基础的荧光定量PCR技术 ,自 1 995年建立以来 ,无论从技术和应用上都得到了快速发展。本文就荧光定量PCR的各种探针技术及其在肿瘤检测方面的应用作一简要综述。
出处 《国外医学(临床生物化学与检验学分册)》 2004年第2期119-121,124,共4页 Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
  • 相关文献

参考文献17

  • 1Tubbs RR,Pettay JD, Roche PC, et al. Discrepancies in clinical laboratory testing of eligibility for trastuzumab therapy:apparent immunohistochemical false-positives do not get the message. J Clin Oncol, 2001,19(10): 2714-2721.
  • 2Seeger K,Kreuzer KA,Lass U,et al. Molecular quantification of response to therapy and remission status in TEL-AMLl-positive childhood ALL by real-time reverse transcription polymerase chain reaction. Cancer Res,2001,61(6) :2517-2522.
  • 3Bieche I,Olivi M,Champeme MH,et al. Novel approach to quantitative polymerase chain reaction using real-time detection:application to the detection of gene amplification in breast cancer. Int J Cancer,1998,78(5) :661-666.
  • 4Jarvinen TA,Tanner M, RantanenV, et al. Amplification and deletion of topoisomerase IIalpha associate with ErbB-2 amplification and affect sensitivity to topoisomerase Ⅱ inhibitor doxorubicin in breast cancer. Am J Pathol,2000,156(3) :839-847.
  • 5Paradis V,Bieche I,Dargere D,et al. hTERT expression in sporadic renal cell carcinomas. J Psthol,2001,195(2) :209-217.
  • 6Faneyte IF, Kristel PM, van-de-Vijver MJ. Determining MDR1/Pglycoprotein expression in breast cancer. Int J Cancer,2001,93(1):114-122.
  • 7Whitcombe D,Theaker J,Guy SP,et al. Detection of PCR products using self-probing amplicons and fluorescence. Nat Biotechnol,1999,17(8) :804-807.
  • 8Mitas M, Mikhitarian K, Walters C, et al. Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multigene marker panel. Int J Cancer,2001,93(2) :162-171.
  • 9Jarvinen TA,Tanner M,Barlund M,et al. Characterization of topoisomerase II alpha gene amplification and deletion in breast cancer.Genes Chromosomes Cancer, 1999,26(2): 142-150.
  • 10Bernard PS,Wittwer CT. Real-time PCR technology for cancer diagnostics. Clin Chem,2002,48(8) :1178-1185.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部