期刊文献+

RNA干涉技术

下载PDF
导出
摘要 RNA干涉 (RNAi)是在动物和植物中由双链RNA(dsRNA)引发的序列特异性的转录后基因沉默。它不但是基因功能分析的重要手段 ,还可以用于疾病治疗。本文就RNAi的作用机制、作用相关的酶。
出处 《国外医学(临床生物化学与检验学分册)》 2004年第2期172-173,177,共3页 Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
  • 相关文献

参考文献9

  • 1Sonoda S, Nishiguchi M. Graft transmission of post-transcriptional gene silencing:target specificity for RNA degradation is transmissible between silenced and non-silenced plants, but not between si lenced plants. Plant J, 2000,21(1 ): 1-8.
  • 2Fire A,Xu S,Montgomery MK,et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditiselegans. Nature, 1998,391(6669) :806-811.
  • 3Jiang M, Milner J. Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA,a primer of RNA interference. Oncogene, 2002,21 (39): 6041-6048.
  • 4Bernstein E,Caudy AA,Hammond SM,et al. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature,2001,409(6818) :363-366.
  • 5Jacobsen SE,Running MP,Meyerowitz EM. Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems. Development, 1999,126(23): 5231-5143.
  • 6Sijen T,Fleenor J, Simmer F, et al. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell, 2001, 107 (4) : 465-476.
  • 7Brummelkamp TR,Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science,2002,296(5567) :550-553.
  • 8Maeda I,Kohara Y,Yamamoto M,et al. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr Biol, 2001,11 (3): 171-176.
  • 9Capodici J,Kariko K,Weissman D. Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference. J Immunol,2002,169(9): 5196-5201.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部