当归补血浸膏的定性定量分析

Qualitative and quantitative analysis of Danggui Buxue Jingao

目的 快速简便的鉴别当归补血浸膏剂中的当归与党参、甘草与芍药。方法 用HPLC方法测定制剂中芍药苷和甘草酸的含量。薄层色谱法鉴别 4味药材。结果 薄层色谱上均鉴别出制剂和各自药材的特征斑点 ,而空白对照液无此斑点。高效液相色谱方法的建立 ,相关系数分别为芍药苷r=0 .9999,甘草酸r=0 .9996 ,平均回收率芍药苷 99.5 4 % ,RSD1.11%。甘草酸 98.79% ,RSD0 .6 0 %。结论 方法简便 ,结果准确。

OBJECTIVE To identify Danggui, danshen, gancao and baoshao, and determine the contents of paeonilorin and glycyrrhizic acid in Danggui buxui jingao. METHOD TLC and HPLC method were applied to identification and quantitative analysis, respectively.RESULTS The characteristic dots of four medicinal materials were found on TLC chromatogram of sample, however they were not shown in negative sample. The regression coefficient for determination of paeonilorin and glycyrrhizic acid by HPLC were 0.9999 and 0.9996, and the average recovery were 99.54%(RSD=1.11%) and 98.79% (RSD=0.60%), respectively.CONCLUSION The methods developed are simple and accurate.