益母草治疗痛经机制探索

Effects of motherwort on dysmenorrhea

目的 探讨益母草治疗痛经的药理作用及机制。方法 经十二指肠给予益母草水提液 ,观察豚鼠在体子宫收缩频率及幅度的变化。分别采用由缩宫素及 15甲基 PGF2α所致的子宫痉挛模型 ,观察小鼠口服益母草提取液后的作用。采用二甲苯致小鼠耳廓肿胀及实验性大鼠子宫炎症模型 ,分别观察小鼠、大鼠口服益母草提取液的抗炎作用。用放射免疫法及化学分析法分别检测大鼠口服益母草水提液后血液雌、孕激素及子宫平滑肌PGF2α及PGE2 含量的变化。结果 益母草水提液能增强未孕正常豚鼠在体子宫的收缩。益母草能在一定程度上缓解由 15M PGF2α及缩宫素所致的小鼠子宫痉挛 ,能减轻二甲苯所致的小鼠耳廓肿胀的程度 ,改善实验性子宫炎症状况 ,且能降低子宫炎症时其平滑肌上PGE2 的含量 ,亦能降低大鼠子宫平滑肌上PGF2α的含量。益母草水提液能升高血液孕激素的水平 ,而对雌激素却无明显影响。结论 益母草对子宫具有比较广泛的药理作用 ,可能通过抑制痉挛子宫的活动、抗炎、降低子宫平滑肌上PGF2α,PGE2 的含量及升高体内孕激素水平等多种途径缓解痛经症状。

OBJECTIVE To investigate the pharmacological actions and mechanisms of motherwort on dysmenorrhea. METHOD In vivo, the effects of motherwort on uterine constriction were observed after giving the decoction via duodenum in guinea pigs. Effect on alleviating pain was measured in mice treated with i.p. administration of oxytocin and 15M-PGF 2α respectively. Anti-inflammatory action was examined with the models of auricular swelling in mice induced by dimethyl benzene, and of the experimental inflammatory uteri in rats. The influence on the concentrations of PGF 2α , PGE 2 in the uterine smooth muscle was measured. The levels of serum estradiol, progesterone in rats were determined by radioimmunoassay.RESULTS The data showed that the motherwort decoction increased the constrictive force of normal uterus in guinea pigs in vivo. It obviously reduced the twisting reaction of mice induced by oxytocin or 15M-PGF 2α .The decoction could alleviate the auricular edema in mice and inflammation of uterus in rats. It reduced PGF 2α and PGE 2 concentrations in uterine smooth muscle of rats. The motherwort decoction increased serum progesterone level in rats, but had no significant effect on estradiol level. CONCLUSION Motherwort has intensive pharmacological effects on uteri. It had the effects on relaxing uterus spasm, anti-inflammation, reducing PGF 2α and PGE 2 concentrations in uterus smooth muscle, increasing the serum progesterone level. These effects may play important roles in treating dysmenorrhea.