摘要
目的 观察NMDA受体和一氧化氮 (NO)对海马脑片突触传递长时程增强 (LTP)产生中神经细胞粘附分子 (NCAM)合成的影响。方法 采用胞外记录方法检测LTP产生和维持。Westernblot技术检测NCAM合成变化。结果 条件刺激 10min后 ,LTP产生 ,NCAM合成显著增加。条件刺激 60min后 ,LTP稳定维持 ,NCAM蛋白水平持续增高。NMDA受体阻断剂AP 5和NO合酶抑制剂N硝基精氨酸在抑制LTP产生的同时 ,也显著抑制NCAM合成。
Objective To observe the effects of NMDA receptors and nitric oxide (NO) on neural cell adhesion molecule(NCAM) synthesis during hippocampal long term potentiation (LTP) induction in vitro . Methods LTP induction and maintenance were tested by using extracelluar electrophysiological recording. The synthesis of NCAM protein was detected by Western blotting. Results Application of conditioned stimulation induced LTP and a rise in NCAM at 10 min. NCAM protein level continued to rise while LTP remained stably at 60 min. The NMDA receptor inhibitor AP 5 and the NO synthase inhibitor N nitro arginine inhibited the LTP induction and the increase in the NCAM synthesis. Conclusion The changes in NCAM synthesis during hippocampal LTP induction in vitro may be involved in NMDA receptors and NO.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第1期43-46,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 396 0 0 0 4 8)~~
关键词
长时程增强
一氧化氮
神经细胞粘附分子
海马
大鼠
long tern potentiation
nitric oxide
neural cell adhesion molecule
hippocampus
rat
