安定-氯胺酮麻醉对烧伤小鼠早期三种炎性细胞因子影响

Diazepam-ketamine inhibits 3 kinds of serum inflammatory cytokines during the early stage in burned mice

目的 研究安定 氯胺酮麻醉对烧伤小鼠早期炎性细胞因子的影响 ,初步探讨其对创伤应激早期炎症反应的调节作用。方法 雄性BALB c小鼠 ,随机分为正常对照组、烧伤对照组、麻醉对照组、预处理组 (麻醉后 15min烧伤 )及后处理组 (烧伤后 15min麻醉 )。麻醉采用安定 氯胺酮麻醉 ,背部 15 %～ 2 0 %Ⅲ度烧伤。烧伤后 4h测定血清TNF α、IL 1β、IL 10浓度。结果 烧伤后血清TNF α、IL 1β、IL 10水平均比正常对照组显著增高 ;预处理组与后处理组血清TNF α、IL 1β、IL 10的浓度明显低于烧伤对照组 ;预处理组除IL 10明显高于正常对照组以外 ,其余均与正常对照组相差不明显 ,而后处理组只有TNF α水平与正常对照组相差不显著 ,其余都显著高于正常对照组 ;血清中IL 1β后处理组明显高于预处理组 ,而TNF α、IL 10的水平两组相差不显著。结论 安定 氯胺酮麻醉对严重创伤后炎性细胞因子的表达和释放有一定抑制作用 。

Objective To evaluate the inhibitory effects of diazepam ketamine on serum inflammatory cytokines during the early stage in burned mice. Methods BALB/c male mice were divided randomly into five groups: control; burn control, being inflicted 15%-20% third degree burn injury; anesthesia control, receiving diazepam ketamine combined anesthesia only; pretreatment, receiving diazepam ketamine combined anesthesia initiated 15 min before burn injury; and posttreatment, receiving diazepam ketamine combined anesthesia initiated 15 min after burn injury. At 4 h after burn injury or anesthesia, levels of serum TNF α, IL 1β, IL 10 were determined by enzyme linked immunosorbant assay. Results After burn injury, levels of serum TNF α, IL 1β, IL 10 in all burn groups were significantly higher than those in the control group. The levels of serum TNF α and IL 1β in pretreatment group was not significantly different from those in the control group except IL 10. TNF α concentration in posttreatment group was not significantly different from that in the control group, but levels of IL 1β and IL 10 were obviously higher than those in the control group. Serum IL 1β in the pretreatment group was markedly lower than that in the post treatment group. Conclusion Diazepam ketamine anesthesia can inhibit the expression and release of cytokines following severe trauma.