成年大鼠坐骨神经切断后脊髓运动神经元死亡方式的研究

Pattern of motoneuron death following transection of the sciatic nerve in adult rats

目的 探索成年大鼠坐骨神经切断损伤后脊髓前角运动神经元的死亡方式。方法 选择大鼠坐骨神经切断损伤模型 ,术后 48h、7d、15d、3 0d动物经 4%多聚甲醛常规灌注固定 ,取L4 ～L6 脊髓节段 ,TUNEL染色和电镜观察。结果 坐骨神经切断后TUNEL染色和电镜观察均检测到脊髓前角运动神经元典型的凋亡形态学改变。结论 周围神经切断后脊髓前角运动神经元出现凋亡 。

Objective To investigate the pattern of motoneuron death induced by transection of the sciatic nerve in adult rats. Methods Sciatic nerve transected model was selected. The rats were sacrificed at 48 h, 7, 15, and 30 d after operation, respectively. The L 4-L 6 segments of the spinal cord were harvested after transcardiac perfusion with 4% paraformaldehyde, and were stained by TUNEL method and examined electron microscopically. Results A typical pattern of morphological changes of motoneuron apoptosis in the anterior horn of spinal cord was observed by both TUNEL staining and electron microscopy. Conclusion Motoneuron apoptosis in the spinal cord anterior horn induced by peripheral nerve transection demonstrates that neuron apoptosis may be the main pathological course of rat spinal cord neuron death following peripheral nerve injury in adult rats.