肺炎支原体16SrRNA基因引物A(201)B(629)多聚酶链式反应特异性和敏感性的研究

THE SPECIFICITY AND SENSETIVITY STUDY OF POLYMERASE CHAIN REACTION (PCR) WITH MP. PNEUMONIAE 16S rRNA GENE SEQUENCES A (201) B (629) AS PRIMERS

用肺炎支原体16S rRNA基因序列A(201)B(629)做引物,以肺炎支原体、生殖支原体、口腔支原体、唾液支原体和人型支原体DNA为模板进行多聚酶链式反应(PCR),检测引物的特异性和敏感性。结果肺炎支原体呈阳性,但与生殖支原体有交叉;可检出标本中肺炎支原体最少菌量小于10~3/ml,敏感性高于核酸杂交。

Polymerase chain reaction was done to test specificity and sensetivity with Mp. pneumoniae 16S rRNA gene sequences A (201) B (629) as primers and DNA from Mp. pneumoniae, Mp. genitalium, Mp. orale, Mp.salivarium and Mp.hominis as templates respectively.Results indicated that Mp.pneumoniae is positive. Although there was cross reaction between Mp. pneumoniae and Mp. genitalium, they could be distinguised by the sources of speciman. The minimum Mp. pneumoniae that could be detected in speciman was less than 10~3/ml and the sensetivity by PCR was higher than that by hybridization. This experiment provided basis for the applification of primers A (201)B (629) in detection of Mp. pneumoniae by PCR.