四川人群中CYP1A1基因Ile-Val和Msp1位点多态性与肺癌易感性的研究

Study on the relationship between polymorphisms of CYP1A1 gene and susceptibility of lung cancer in Sichuan population

目的 探讨细胞色素P45 0 (CYP1A1)基因异亮氨酸 (Ile) 缬氨酸 (Val)位点和Msp1位点多态性和肺癌易感性的相关关系。方法 以病例对照的研究方法 ,采用PCR RFLP和ASA PCR技术检测 82例原发性肺癌和 91例对照的CYP1A1基因Ile Val位点和Msp1位点多态性。 结果 Ile Val三种多态基因型在肺癌组和对照组分布差异有显著性 (P <0 .0 5 ) ,Ile/Val、Val/Val基因型在肺癌组的分布频率明显高于对照组 ;logistic回归分析结果显示Ile/Val、Val/Val基因型患肺癌的危险分别是Ile/Ile基因型的 1.969倍和3 .15 0倍 ;当按吸烟分层后 (将Ile/Val、Val/Val基因型合并分析 ) ,吸烟组中Ile/Val、Val/Val合并基因型患肺癌的危险是Ile/Ile基因型的 3 .0 5 9倍 ,而在不吸烟组其OR为 1.687;Msp1位点多态性在肺癌组和对照组差异无统计学意义。结论 CYP1A1第 7外显子的Ile/Val、Val/Val基因型与肺癌的易感性有关 ,可望作为肺癌易感人群筛选的重要指标 ;

Objective To investigate the CYP1A1 gene Ile Val and Msp1 polymorphisms and theirs relationship with susceptibility of lung cancer in Sichuan population. Methods Ile Val and Msp1 polymorphisms of CYP1A1 were detected in 82 cases of lung cancer and 91 cases of other diseases by PCR RFLP and ASA PCR. Results The frequencies of 7th exon polymorphisms in cancer group and control group were statistically significant different ( P <0.05), the distribution value of Ile/Val and Val/Val genotype in cancer group was significantly higher than that in control group. Logistic regression analysis showed that the risk of Ile/Val and Val/Val to lung cancer was 1.969 and 3.150 times respectively to Ile/Ile genotype. Considering the risk factor of smoking, the risk of lung cancer in individuals with Ile/Val or Val/Val genotypes was 3.059 times of others with Ile/Ile genotype. However, in nonsmoking group OR was 1.678 ( P >0.05). There was no significant correlation between Msp1 polymorphism and lung cancer susceptibility. Conclusion The results suggest that Ile/Val and Val/Val genotypes are related to the susceptibility of lung cancer in smoking subject, they might be one of the risk factors of lung cancer. The results do not show that polymorphism of Msp1 is associated with susceptibility of lung cancer.