氮芥对体外培养HaCaT细胞白细胞介素分泌的影响

The Effect of Mechlorethamine on Cytokines Secretion of Human Keratinocyte HaCaT Cell Line in Vitro

目的 观察氮芥对体外培养的HaCaT细胞细胞因子分泌的影响。方法 佛泊脂 /脂多糖 (PMA/LPS)刺激HaCaT细胞后培养 ,用ELISA法检测HaCaT细胞培养上清液中细胞因子的含量。结果 HaCaT细胞可自发性分泌IL 1α、IL 2 ,IL 6和IL 8。PMA/LPS刺激HaCaT细胞后 ,IL 1α分泌量下降 ,IL 6和IL 8的分泌量明显增加 ,与银屑病患者皮损中细胞因子的变化非常相似。加入氮芥后HaCaT细胞IL 6和IL 8的分泌量增加 ;在低浓度氮芥作用下IL 1α的分泌量无明显变化 ,高浓度时则可使IL 1α的分泌量培加。对于PMA/LPS刺激的HaCaT细胞 ,低浓度氮芥对其IL 6和IL 8的分泌量均有明显抑制作用 ,而IL 1α的分泌明显增加 ;高浓度氮芥则可使IL 1α、IL 6和IL 8的分泌量增加。结论 氮芥治疗银屑病的机制可能与IL 1α、IL 6和IL 8有关。

Objective To Study the effect of mechlorethamine on cytokines secretion of human keratinocyte HaCaT cell line in vitro,both not stimulated and stimulated by PMA/LPS.Methods Expression of IL-1α,IL-2,IL-6 and IL-8 was detected by ELISA kit.Results HaCaT cell secreted IL-1α,IL-2,IL-6 and IL-8 spontaneously;Stimulation by PMA/LPS induced significant increase in IL-6 and IL-8 level and decrease in IL-1α level in HaCaT cell,a pattern similar to those seen in psoriasis.On HaCaT cell,Mechlorethamine increased the secretion of IL-1α,IL-6 and IL-8 distinctly,they may participate in the inflammation of Mechlorethamine on normal skin.On HaCaT cell stimulated with PMA/LPS,Mechlorethamine decreased the secretion of IL-6,IL-8 and increased the secretion of IL-1α in a low concentration,but the secretion of IL-6,IL-8 and IL-1α in stimulated HaCaT cell were significantly increased when Mechlorethamine was in a high concentration.Conclusion These results suggested that the mechanism of Mechlorethamine therapy on psoriasis is related to IL-1α,IL-6 and IL-8.