梅毒螺旋体感染兔模型中特异性抗体分析

Analysis of Specific Antibodies During Treponema Pallidum Infection and Treatment in Rabbit

目的 确立早期诊断梅毒及判定临床疗效的抗体指标。方法 试用梅毒兔模型 ,采用RPR法、TPPA法及免疫印迹法检测梅毒螺旋体感染兔模型治疗前后血清抗体的变化情况。结果 梅毒螺旋体接种入兔机体后 ,3天就可用免疫印迹法检测到血清中 47kD特异性抗原抗体 ,之后相继出现 45kD、17kD、3 7kD、92kD及 15 .5kD抗原的抗体 ,其中45kD、15 .5kD和 92kD抗原的抗体在治疗后逐渐减弱并可消失 ;RPR法检测的心磷脂抗体滴度在感染早期 1～ 1.5个月可随治疗明显下降 ;TPPA法检测到血清中TP抗体较 47kD特异性抗原抗体出现晚 ,且出现的高峰时间及变化的时间均较心磷脂抗体晚。结论 免疫印迹法检测血清 47kD抗原抗体可作为梅毒的早期诊断指标 ,45kD、15 .5kD、92kD抗原抗体和心磷脂抗体在治疗中变化明显 ,可考虑作为疗效观察的指标。

Objective To detect antibodies to treponema pallidum for the early accurate diagnosis of syphilis and direction of clinical treatment.Methods The model of rabbit’s syphilis was founded.By using RPR,TPPA and western immunoblotting technique (WIB),the change of antibodies to treponema pallidum after infection and treatment was tested. Results A positive antibody of molecular 47kD was detected in serum infected rabbit in 3 days by using WIB,and then the specificities of 45kD,17kD,15.5kD,and 92kD-protein antibody were detected.Having been finished treatment,45kD,15.5kD, and 92kD protein antibodies of some rabbits were gradually weak and disappear.Using RPR for syphilis,positive serologic phosphatidic antibodies titers were gradually reduced with treatment in 1-1.5 month after infection.Positive serologic antibodies were later detected by using TPPA than by WIB,Fastigium and change of time of TP antibodies was later than phosphatidic antibodies.Conclusion This study demonstrates that 47kD serum antibodies detected by WIB could be used for the early accurate serum test diagnosis of syphilis;the change of 45kD,15.5kD,and 92kD protein antibodies by using WIB and the change of positive serologic titers by using RPR may be direction of clinical treatment for syphilis.