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基质金属蛋白酶在孔源性视网膜脱离视网膜下液的定量研究 被引量:8

Quantitative study on matrix metalloproteinases of subretinal fluid in rhegmatogenous retinal detachment
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摘要 目的 研究孔源性视网膜脱离 (RRD)视网膜下液 (SRF)基质金属蛋白酶 (MMPs)的表达 ,探讨MMPs与增生性玻璃体视网膜病变 (PVR)的关系。方法 采用明胶酶谱分析法定量检测 88例RRD患者的SRFMMP 2和MMP 9的活性水平 ,PVRA组 2 1例 ,PVRB组 3 7例 ,PVRC组 3 0例 ,其中包括伴有脉络膜脱离RRD 8例 ,术后随访PVR复发 11例。结果  88例RRD患者的SRF均有MMP 2活性水平升高 ,3 3例有MMP 9的表达 ,8例RRD伴有脉络膜脱离 ,有 6例 ( 75 % )MMP 9活性水平升高 ,术后PVR复发 11例SRF均有MMP 9活性水平升高。结论 RRD患者的SRF有MMP 2和MMP 9的表达 ,MMP 9活性水平升高可能与PVR的发生有关 ,可能成为预测术后PVR复发的指标之一。 ObjectiveTo investigate the expression of matrix metalloproteinases (MMPs) in subretinal fluid(SRF) in the eyes with rhegmatogenous retinal detachment (RRD), and evaluate the relationship of MMPs and proliferative vitreoretinopathy (PVR).MethodsActivities of MMP-2 and MMP-9 were determined by quantitative gelatinase zymography in 88 patients with RRD, 21 patients with PVRA, 37 patients with PVRB and 30 patients with PVRC, including 8 cases with choroidal detachment and 11 cases with postoperative recurrence PVR.ResultsMMP-2 was detected in all of SRF samples, whereas MMP-9 was found in 33 of 88 SRF with RRD and 6(75%) of 8 RRD with choroidal detachment. Eleven patients whose MMP-9 level elevated developed postoperative PVR.ConclusionMMP-2 and MMP-9 were found in the SRF of RRD. A rising in MMP-9 may be one of important predictors for postoperative PVR recurrence.
出处 《眼科研究》 CSCD 北大核心 2004年第2期170-172,共3页 Chinese Ophthalmic Research
关键词 基质金属蛋白酶 孔源性视网膜脱离 视网膜下液 定量研究 matrix metalloproteinases rhegmatogenous retinal detachment proliferative vitreoretinapathy subretinal fluid
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