全反式维A酸对UVA诱导人成纤维细胞胶原合成的影响

Effect of All-trans Retinoic Acid on UVA Induced Collagen Biosynthesis in Cultured Human Normal Fibroblast

目的 探讨全反式维A酸 (at RA)对UVA诱导人成纤维细胞胶原生物合成的影响 ,为应用全反式维A酸治疗光老化皮肤病提供实验依据。方法 正常人来源的单层培养的皮肤成纤维细胞 ,经UVA照射后立即加入含不同浓度at RA的培养液 ,共 2天 ,用MTT法检测细胞增殖情况 ,3 H 脯氨酸检测细胞胶原合成情况。结果 在各组之间细胞增殖差别无显著性时 ,at RA浓度为 10 6mmol/L和 10 -5mmol/L的实验组比对照组的细胞胶原合成显著增高 (P <0 .0 5 ,P <0 .0 1) ;而at RA浓度为 10 -4mmol/L的实验组比对照组的细胞胶原合成显著降低 (P <0 .0 1)。结论 在体外细胞培养情况下 ,相对低浓度的at RA(10 -6mmol/L ,10 -5mmol/L)可逆转UVA诱导人成纤维细胞胶原生物合成的降低 ,而相对高浓度 (10 -4mmol/L)

Objective To investigate the effects of all-trans retinoic acid(at-RA) on UVA-induced collagen biosynthesis of human normal fibroblasts and to provide experimental evidence for treating photoaged skin.Methods Fibroblasts monolayers from normal human skin cultured in vitro were irradiated by UVA light and added different concentration of at-RA immediately after each irradiation.Two days after the irradiation,the cell’s proliferation was measured by MTT method,and the collagen biosynthesis was measured by 3H-proline incorporation.Results There was no statistical difference of cell’s proliferation between the different concentration of at-RA groups and vehicle group,the collagen biosynthesis of fibroblasts treated by relatively low concentration of at-RA(10 -6 mmol/L,10 -5 mmol/L) group was increased than those treated by vehicle( P<0.05,P <0.01),but the relatively high concentration of at-RA(10 -4 mmol/L) group,on contrast,was decreased( P <0.01).Conclusion The at-RA at relatively low concentration can reverse the UVA induced the fibroblasts collagen biosynthesis decreasing in vitro,but not at relatively high concentration.