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传染性软疣病毒MC148基因的克隆和序列分析 被引量：1

Cloning and analysis of MC148 gene of molluscum contangiosum virus
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摘要 目的 :克隆传染性软疣病毒 (MCV)的MC14 8基因 ,研究其基因序列。　方法 :从传染性软疣患者皮肤上的软疣小体中提取病毒DNA ,经PCR扩增并测序后构建于 pUC19克隆载体上。对重组体pUC19 MC14 8再次进行测序。　结果 :扩增出约 315bp的片段 ,PCR产物及克隆产物测序证明 ,基因序列与GenBank报道的相同。　结论 :成功克隆出MCV的MC14 8基因 ,为趋化因子拮抗剂的研究奠定基础。 Objective:To clone MC148 gene of molluscum contagiosum virus(MCV). Methods:MCV DNA was extracted from the specimens of patients with multiple molluscum lesions and amplified. The product was then cloned into vector pUC19 and sequenced . Result:The sequence of MC148 gene with 315bp cloned was identical to that reported in GenBank. Conclusion:The MCV MC148 gene was cloned successfully.
作者 孙星慧 谭建明 严伟 吴卫真 周浩 林文洪
机构地区 南京军区福州总医院泌尿外科
出处 《医学研究生学报》 CAS 2004年第4期300-301,314,共3页 Journal of Medical Postgraduates
关键词 趋化因子 传染性软疣病毒 基因克隆 聚合酶链反应 Gene cloning Molluscum contagiosum virus Chemokine PCR
分类号 R392.12 [医药卫生—免疫学]
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共引文献8

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  • 2陈翔,马保安,杨彤涛,张勇,杨辉,范清宇.人存活素基因编码区的克隆和序列分析[J].医学研究生学报,2005,18(1):17-19. 被引量：1
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医学研究生学报
2004年 第4期

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