同型半胱氨酸刺激大鼠动脉平滑肌细胞增生功能蛋白质组的初步研究

The functional proteome preliminary study of rat arterial smooth muscle cells promoted by homocysteine

目的 :通过同型半胱氨酸 (Hcy)刺激前后大鼠动脉平滑肌细胞中蛋白质表达的变化 ,探讨Hcy引起动脉平滑肌细胞增生的机制。 方法 :对大鼠动脉平滑肌细胞进行原代培养 ,取Hcy刺激前后的细胞提取蛋白质 ,利用二维电泳的方法得到细胞蛋白质电泳图谱 ,通过计算机图像分析系统进行分析。 结果 :Hcy刺激前后 ,大鼠动脉平滑肌细胞中有 2 8种蛋白质发生了改变 ,其中有 11种在刺激后表达量增加 ,1种表达量减少 ,4种刺激后消失 ,12种在刺激后新出现。 结论 :Hcy刺激后大鼠动脉平滑肌细胞中蛋白质的表达发生改变 ,考虑这些蛋白质与平滑肌细胞增生有关。

Objective:We investigated the changes of the proteins in the rat arterial smooth muscle cells induced by homocysteine.Detected the mechanism by which homocysteinemia leads to atherosclerosis. Methods:The rat arterial smooth muscle cells of primary culture were planned to be the experimental systems.Extracted the proteins from the smooth muscle cells which were induced by homocysteine and control.After 2-D electrophoresis,the map was analyzed by imaging systems. Results:After cells promoted by homocysteine, twenty-eight proteins of the smooth muscle cells changed.Twelve proteins were found to differ in quantity(11 higher and 1 lower).Sixteen proteins were seen to be unique in one region or the others(12 points of protein appear after Hcy stimulating,4 points of protein disapear after Hcy stimulating). Conclusion:The protein of rat arterial muscle cells changed after promoted by homocysteine.We considered that the proteins were related to the proliferation of the smooth muscle cells.