ANF和ACTH对体外培养的人正常肾上腺组织分泌醛固酮的影响

Effects of ANF and ACTH on Aldosterone Secretion in Cultured Human Adrenal Tissue

将体外培养的人正常肾上腺组织,用不同浓度的心钠素(atrial natriuretic factor,ANF)和ACTH作用不同时间,观察其醛固酮分泌的变化。结果表明:ANF10~mol/L作用0.5h能刺激正常肾上腺组织分泌醛固酮,而作用24h则呈现抑制作用;同浓度ANF能抑制同时有ACTH10^(-8)mol/L参与作用的醛固酮分泌。ACTH10^(-8)moL/L单独作用不能刺激正常肾上腺组织分泌醛固酮,当浓度增加到3×10^(-8)或5×10^(-8)mol/L时才能使醛固酮分泌增加。以相同浓度的ANF和ACTH(3×10^(-8)或5×10^(-8)mol/L)同时作用于肾上腺组织,ANF不能完全抑制由ACTH引起的醛固酮分泌增加的作用。提示体外培养的正常肾上腺组织分泌醛固酮的反应与ANF和ACTH的剂量及作用时间有关。

We studiedthe effects of various concentrations of ANF and ACTH on aldosterone secretion in human normal adrenal tissue over different response times in vitro. We found that ANF 10^(-8) mol/L at first stimulated but 24 h later inhibited basal aldosterone secretion. ANF 10^(-8) mol/L also inhibited the ACTH-stimulated secretion of aldosterone. ACTH 10^(-8) mol/L did not stimulate aldosterone secretion, while ACTH concentrations of 3×10^(-8) and 5×10^(-8) mol/L did. When ACTH and ANF were added simultaneously, the ANF-induced inhibition of ACTH-stimulated aldosterone secretion could be overcome by increasing the concentration of ACTH. Our results show that ANF caused a dose-dependent and time-dependent inhibition of basal and ACTH-stimulated increases in aldosterone secretion.