人成釉细胞瘤中端粒酶活性与细胞周期素A的表达

Expression of telomerase activity and cyclin A in ameloblastoma

目的 研究端粒酶hTERT基因在人成釉细胞瘤 (ameloblastoma,AB)中的表达及其与细胞周期素A (cyclinA)和相关因子 p53蛋白、增殖细胞核抗原 (proliferationcellnuclearantigen ,PCNA)的关系 ,探讨其临床生物学意义。方法 用免疫组化 (SP法 )和原位杂交技术分别检测了AB中细胞周期素A、p53蛋白和PCNA ,hTERTmRNA的表达 ,同时以正常口腔粘膜和牙源性角化囊肿(odontogenickeratocyst,OKC)为对照。 结果 在正常口腔粘膜中只有 1例hTERT阳性 (1 / 7) ,cyclinA、p53蛋白、PCNA三者之间表达有着相似共性。OKC中表达在基底或副基底层细胞核中 ,有1 4例hTERT阳性 (1 4 / 1 6) ,4例cyclinA阳性 (4/ 32 )、1 1例p53蛋白阳性 (1 1 / 2 5) ,5例PCNA阳性(5/ 9)。hTERTmRNA在AB中表达率为 94 4% (51 / 54) ,cyclinA为 66 7% (40 / 60 ) ,p53蛋白为85 7% (42 / 4 9) ,PCNA为 78 1 % (2 5/ 32 ) ,hTERT与cyclinA、p53蛋白、PCNA之间经Kendall相关分析 ,rs 分别为 0 91 4、0 848、0 80 4 ,P <0 0 5。结论 随着组织学分级增高 ,hTERTmRNA与cyclinA、p53蛋白、PCNA阳性率及信号强度均增高。在AB中端粒酶的活性表达与p53蛋白的蓄积有关 ,与细胞的增殖、分化有关 ,并受cyclinA调节 。

Objective To study relation of the expression of hTERT mRNA and cyclin A, p53 protein, proliferation cell nuclear antigen (PCNA)in ameloblastoma (AB) and to investigate clinical biological characteristics of AB Methods The hTERT mRNA was detected by in situ hybridization, cyclin A , p53 protein and PCNA by SP method Normal oral mucosa and odontogenic keratocyst(OKC) are comparition Results The positive ratio of hTERT mRNA was 1/7 cases in normal oral mucosa The expression of cyclin A, p53 and PCNA in normal oral mucosa were similar, and the positive cells distributed in stratum basale In OKC, the positive cells distributed in stratum basale and super strrtum basale And the positive ratio of hTERT, cyclin A, p53, PCNA in OKC was 14/16 cases, 4/32 cases, 11/25 cases, 5/9 cases, respectively In AB, the positive ratio of hTERT mRNA, cyclin A, p53 protein and PCNA was 94 4%(51/54), 66 7%(40/60), 85 7%(42/49) and 78 1%(25/32), respectively A strong correlation was found between hTERT mRNA with cyclin A, p53 protein and PCNA ( r s =0 914, 0 848, 0 804, P <0 05) Conclusions The expression of hTETR mRNA, cyclin A, p53 and PCNA is different in different tissues and lesions, and correlates with cell differentiation and clinical biology behaviour Telomerase activity is related to cumulation of p53 protein, related to cell proliferation and differentiation, regulated by cyclin A, and higher in S phase